Inactivation of α2-Macroglobulin by Activated Human Polymorphonuclear Leukocytes
The proteolytic activity of trypsin releases the dye Remazol Brilliant Blue from its high molecular weight substrate, the skin powder (Hide Powder Azure, Sigma), with an increase in absorbance at 595 nm. Active α2- macroglobulin (80 μg/ml) totally inhibits the proteolytic activity of trypsin (14 μg/...
Main Authors: | , , , , , , , |
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Format: | Article |
Language: | English |
Published: |
Hindawi Limited
1994-01-01
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Series: | Mediators of Inflammation |
Online Access: | http://dx.doi.org/10.1155/S0962935194000141 |