ELECTROPORATION OF CONFLUENT HM-1 ES CELLS LEADS TO HIGHER AMOUNT COLONIES

Electroporation is used to induce homologous recombination in the genome of the murine ES (embryonic stem) cells. Routinelly subconfluent ES cells are recommended to be used in such experiments. Electroporation of immunoglobulin specific targeting vectors with different length of homology leads to r...

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Main Authors:	A.ALENA BENCSIK, I. BENCSIK, JANA STĂNCULEł, M. BURA
Format:	Article
Language:	English
Published:	Agroprint Timisoara 2008-05-01
Series:	Scientific Papers Animal Science and Biotechnologies
Subjects:	hm-1 embryonic stem cells colony number confluent subconfluent selection
Online Access:	http://spasb.ro/index.php/spasb/article/view/1146

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spelling	doaj-3119b849f29a4d06b8c1631923a9640c2020-11-25T03:59:26ZengAgroprint TimisoaraScientific Papers Animal Science and Biotechnologies1841-93642344-45762008-05-0141111151068ELECTROPORATION OF CONFLUENT HM-1 ES CELLS LEADS TO HIGHER AMOUNT COLONIESA.ALENA BENCSIK0I. BENCSIK1JANA STĂNCULEł2M. BURA3Faculty of Animal Sciences and Biotechnologies, TimisoaraFaculty of Animal Sciences and Biotechnologies, TimisoaraFaculty of Animal Sciences and Biotechnologies, TimisoaraFaculty of Animal Sciences and Biotechnologies, TimisoaraElectroporation is used to induce homologous recombination in the genome of the murine ES (embryonic stem) cells. Routinelly subconfluent ES cells are recommended to be used in such experiments. Electroporation of immunoglobulin specific targeting vectors with different length of homology leads to reduced number of selected colonies. The enrichment of double selected colonies is high and thus the amount of HM-1 ES cell colonies for the analysis is very low. Here we show that the electroporation of confluent HM-1 ES cells leads to an increased amount of simple and double selected colonies.http://spasb.ro/index.php/spasb/article/view/1146hm-1embryonic stem cellscolony numberconfluentsubconfluentselection
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	A.ALENA BENCSIK I. BENCSIK JANA STĂNCULEł M. BURA
spellingShingle	A.ALENA BENCSIK I. BENCSIK JANA STĂNCULEł M. BURA ELECTROPORATION OF CONFLUENT HM-1 ES CELLS LEADS TO HIGHER AMOUNT COLONIES Scientific Papers Animal Science and Biotechnologies hm-1 embryonic stem cells colony number confluent subconfluent selection
author_facet	A.ALENA BENCSIK I. BENCSIK JANA STĂNCULEł M. BURA
author_sort	A.ALENA BENCSIK
title	ELECTROPORATION OF CONFLUENT HM-1 ES CELLS LEADS TO HIGHER AMOUNT COLONIES
title_short	ELECTROPORATION OF CONFLUENT HM-1 ES CELLS LEADS TO HIGHER AMOUNT COLONIES
title_full	ELECTROPORATION OF CONFLUENT HM-1 ES CELLS LEADS TO HIGHER AMOUNT COLONIES
title_fullStr	ELECTROPORATION OF CONFLUENT HM-1 ES CELLS LEADS TO HIGHER AMOUNT COLONIES
title_full_unstemmed	ELECTROPORATION OF CONFLUENT HM-1 ES CELLS LEADS TO HIGHER AMOUNT COLONIES
title_sort	electroporation of confluent hm-1 es cells leads to higher amount colonies
publisher	Agroprint Timisoara
series	Scientific Papers Animal Science and Biotechnologies
issn	1841-9364 2344-4576
publishDate	2008-05-01
description	Electroporation is used to induce homologous recombination in the genome of the murine ES (embryonic stem) cells. Routinelly subconfluent ES cells are recommended to be used in such experiments. Electroporation of immunoglobulin specific targeting vectors with different length of homology leads to reduced number of selected colonies. The enrichment of double selected colonies is high and thus the amount of HM-1 ES cell colonies for the analysis is very low. Here we show that the electroporation of confluent HM-1 ES cells leads to an increased amount of simple and double selected colonies.
topic	hm-1 embryonic stem cells colony number confluent subconfluent selection
url	http://spasb.ro/index.php/spasb/article/view/1146
work_keys_str_mv	AT aalenabencsik electroporationofconfluenthm1escellsleadstohigheramountcolonies AT ibencsik electroporationofconfluenthm1escellsleadstohigheramountcolonies AT janastanculeł electroporationofconfluenthm1escellsleadstohigheramountcolonies AT mbura electroporationofconfluenthm1escellsleadstohigheramountcolonies
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ELECTROPORATION OF CONFLUENT HM-1 ES CELLS LEADS TO HIGHER AMOUNT COLONIES

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