ELECTROPORATION OF CONFLUENT HM-1 ES CELLS LEADS TO HIGHER AMOUNT COLONIES
Electroporation is used to induce homologous recombination in the genome of the murine ES (embryonic stem) cells. Routinelly subconfluent ES cells are recommended to be used in such experiments. Electroporation of immunoglobulin specific targeting vectors with different length of homology leads to r...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
Agroprint Timisoara
2008-05-01
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Series: | Scientific Papers Animal Science and Biotechnologies |
Subjects: | |
Online Access: | http://spasb.ro/index.php/spasb/article/view/1146 |