Development and validation of an rDNA operon based primer walking strategy applicable to de novo bacterial genome finishing.

Advances in sequencing technology have drastically increased the depth and feasibility of bacterial genome sequencing. However, little information is available that details the specific techniques and procedures employed during genome sequencing despite the large numbers of published genomes. Shotgu...

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Bibliographic Details
Main Authors: Alexander William Eastman, Ze-Chun eYuan
Format: Article
Language:English
Published: Frontiers Media S.A. 2015-01-01
Series:Frontiers in Microbiology
Subjects:
Online Access:http://journal.frontiersin.org/Journal/10.3389/fmicb.2014.00769/full