High-throughput single-cell chromatin accessibility CRISPR screens enable unbiased identification of regulatory networks in cancer

High-throughput single-cell chromatin accessibility CRISPR screens enable unbiased identification of regulatory networks in cancer

Transcription factor binding dynamics can drive epigenetic states, enabling a diversity of phenotypes. Here the authors present Spear-ATAC to quantify and map perturbations to chromatin accessibility in single cells at high throughput.

Bibliographic Details
Main Authors: Sarah E. Pierce, Jeffrey M. Granja, William J. Greenleaf
Format: Article
Language:English
Published: Nature Publishing Group 2021-05-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-021-23213-w
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spelling doaj-304468b61e004570b0aa5b787c82da4e2021-05-23T11:12:07ZengNature Publishing GroupNature Communications2041-17232021-05-011211810.1038/s41467-021-23213-wHigh-throughput single-cell chromatin accessibility CRISPR screens enable unbiased identification of regulatory networks in cancerSarah E. Pierce0Jeffrey M. Granja1William J. Greenleaf2Department of Genetics, Stanford University School of MedicineDepartment of Genetics, Stanford University School of MedicineDepartment of Genetics, Stanford University School of MedicineTranscription factor binding dynamics can drive epigenetic states, enabling a diversity of phenotypes. Here the authors present Spear-ATAC to quantify and map perturbations to chromatin accessibility in single cells at high throughput.https://doi.org/10.1038/s41467-021-23213-w
collection DOAJ
language English
format Article
sources DOAJ
author Sarah E. Pierce
Jeffrey M. Granja
William J. Greenleaf
spellingShingle Sarah E. Pierce
Jeffrey M. Granja
William J. Greenleaf
High-throughput single-cell chromatin accessibility CRISPR screens enable unbiased identification of regulatory networks in cancer
Nature Communications
author_facet Sarah E. Pierce
Jeffrey M. Granja
William J. Greenleaf
author_sort Sarah E. Pierce
title High-throughput single-cell chromatin accessibility CRISPR screens enable unbiased identification of regulatory networks in cancer
title_short High-throughput single-cell chromatin accessibility CRISPR screens enable unbiased identification of regulatory networks in cancer
title_full High-throughput single-cell chromatin accessibility CRISPR screens enable unbiased identification of regulatory networks in cancer
title_fullStr High-throughput single-cell chromatin accessibility CRISPR screens enable unbiased identification of regulatory networks in cancer
title_full_unstemmed High-throughput single-cell chromatin accessibility CRISPR screens enable unbiased identification of regulatory networks in cancer
title_sort high-throughput single-cell chromatin accessibility crispr screens enable unbiased identification of regulatory networks in cancer
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2021-05-01
description Transcription factor binding dynamics can drive epigenetic states, enabling a diversity of phenotypes. Here the authors present Spear-ATAC to quantify and map perturbations to chromatin accessibility in single cells at high throughput.
url https://doi.org/10.1038/s41467-021-23213-w
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AT jeffreymgranja highthroughputsinglecellchromatinaccessibilitycrisprscreensenableunbiasedidentificationofregulatorynetworksincancer
AT williamjgreenleaf highthroughputsinglecellchromatinaccessibilitycrisprscreensenableunbiasedidentificationofregulatorynetworksincancer
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