Etiology of Severe Pneumonia in Children in Alveolar Lavage Fluid Using a High-Throughput Gene Targeted Amplicon Sequencing Assay

Etiology of Severe Pneumonia in Children in Alveolar Lavage Fluid Using a High-Throughput Gene Targeted Amplicon Sequencing Assay

Objective: To evaluate the diagnostic value of a high-throughput gene targeted amplicon sequencing (TAS) assay for detecting pathogenic microorganisms in alveolar lavage fluid (ALF) from children with severe community-acquired pneumonia (SCAP).Methods: A retrospective study was performed on 48 froze...

Full description

Bibliographic Details
Main Authors:	Fei Li, Yin Wang, Yuhan Zhang, Peng Shi, Linfeng Cao, LiYun Su, Qiguo Zhu, Libo Wang, Roujian Lu, Wenjie Tan, Jun Shen
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2021-06-01
Series:	Frontiers in Pediatrics
Subjects:	high-throughput sequencing targeted amplicon sequencing multiplex polymerase chain reaction severe community-acquired pneumonia alveolar lavage fluid children
Online Access:	https://www.frontiersin.org/articles/10.3389/fped.2021.659164/full

Similar Items

Ultrahigh-Throughput Multiplexing and Sequencing of >500-Base-Pair Amplicon Regions on the Illumina HiSeq 2500 Platform
by: Johanna B. Holm, et al.
Published: (2019-02-01)

Bacterial Contamination of Plant in vitro Cultures in Commercial Production Detected by High‑Throughput Amplicon Sequencing
by: Dorota Tekielska, et al.
Published: (2019-01-01)

Management of Alveolar Proteinosis by Bronchopulmonary lavage under Extra Corporeal Membrane Oxygenation (ECMO)
by: Tahereh Parsa, et al.
Published: (2015-12-01)

Cytopathological examination of bronchoalveolar lavage fluid in diagnosis of pulmonary alveolar proteinosis
by: Manjari Kishore, et al.
Published: (2018-01-01)

pTrimmer: An efficient tool to trim primers of multiplex deep sequencing data
by: Xiaolong Zhang, et al.
Published: (2019-05-01)

Pulmonary Alveolar Proteinosis with Respiratory Failure-Anaesthetic Management of Whole Lung Lavage
by: Sunita Nandkumar, et al.
Published: (2009-01-01)

Whole lung tissue is the preferred sampling method for amplicon-based characterization of murine lung microbiota
by: Jennifer M. Baker, et al.
Published: (2021-05-01)

A high-throughput amplicon-based method for estimating outcrossing rates
by: Friederike Jantzen, et al.
Published: (2019-05-01)

Whole lung lavage for pulmonary alveolar proteinosis
by: Jayaraman S, et al.
Published: (2010-01-01)

PVAmpliconFinder: a workflow for the identification of human papillomaviruses from high-throughput amplicon sequencing
by: Alexis Robitaille, et al.
Published: (2020-06-01)

An amplicon-based sequencing approach for the study of aeromycology
by: Hamza Mbareche, et al.
Published: (2018-10-01)

Manual chest physiotherapy during whole-lung lavage in pulmonary alveolar proteinosis
by: Umut Bahcaci, et al.
Published: (2021-01-01)

Use of the melting curve assay as a means for high-throughput quantification of Illumina sequencing libraries
by: Hiroshi Shinozuka, et al.
Published: (2016-08-01)

Apical periodontitis: preliminary assessment of microbiota by 16S rRNA high throughput amplicon target sequencing
by: Federico Mussano, et al.
Published: (2018-04-01)

Natrix: a Snakemake-based workflow for processing, clustering, and taxonomically assigning amplicon sequencing reads
by: Marius Welzel, et al.
Published: (2020-11-01)

Computational Analysis of AmpSeq Data for Targeted, High-Throughput Genotyping of Amplicons
by: Jonathan Fresnedo-Ramírez, et al.
Published: (2019-05-01)

Lavagem pulmonar total: A propósito de quatro casos proteinose alveolar Whole lung lavage: Report of four cases of alveolar proteinosis
by: M Aguiar, et al.
Published: (2009-01-01)

Detection of Bacterial Pathogens from Broncho-Alveolar Lavage by Next-Generation Sequencing
by: Stefano Leo, et al.
Published: (2017-09-01)

Diagnostic efficacy of broncho-alveolar lavage carcino-embronic antigen in carcinoma of lung
by: Sudarsan Pothal, et al.
Published: (2019-01-01)

Molecular epidemiology of Plasmodium falciparum by multiplexed amplicon deep sequencing in Senegal
by: Tolla Ndiaye, et al.
Published: (2020-11-01)

An Uncommon Procedure for a Rare Ailment: Massive Bronchoalveolar Lavage in a Patient with Pulmonary Alveolar Proteinosis
by: Belgin Samurkaşoğlu, et al.
Published: (2012-09-01)

NGSpeciesID: DNA barcode and amplicon consensus generation from long‐read sequencing data
by: Kristoffer Sahlin, et al.
Published: (2021-02-01)

Elucidating cryptic dynamics of Theileria communities in African buffalo using a high‐throughput sequencing informatics approach
by: Caroline K. Glidden, et al.
Published: (2020-01-01)

Ultra-accurate microbial amplicon sequencing with synthetic long reads
by: Benjamin J. Callahan, et al.
Published: (2021-06-01)

Design and application of a novel two-amplicon approach for defining eukaryotic microbiota
by: Ana Popovic, et al.
Published: (2018-12-01)

Serial bronchoscopic lung lavage in pulmonary alveolar proteinosis under local anesthesia
by: K Rennis Davis, et al.
Published: (2015-01-01)

Microbiota analysis optimization for human bronchoalveolar lavage fluid
by: Pierre H. H. Schneeberger, et al.
Published: (2019-10-01)

The Grapevine and Wine Microbiome: Insights from High-Throughput Amplicon Sequencing
by: Horatio H. Morgan, et al.
Published: (2017-05-01)

Cross-comparison of exome analysis, next-generation sequencing of amplicons, and the iPLEX® ADME PGx Panel for pharmacogenomic profiling
by: Eng Wee eChua, et al.
Published: (2016-01-01)

Unraveling the Diversity of Eukaryotic Microplankton in a Large and Deep Perialpine Lake Using a High Throughput Sequencing Approach
by: Nico Salmaso, et al.
Published: (2020-05-01)

The effect of reverse transcription enzymes and conditions on high throughput amplicon sequencing of the 16S rRNA
by: Adam Šťovíček, et al.
Published: (2019-10-01)

Fast and Simple Analysis of MiSeq Amplicon Sequencing Data with MetaAmp
by: Xiaoli Dong, et al.
Published: (2017-08-01)

PacBio Amplicon Sequencing Method To Measure Pilin Antigenic Variation Frequencies of Neisseria gonorrhoeae
by: Egon A. Ozer, et al.
Published: (2019-10-01)

Diagnosing and tracing the pathogens of infantile infectious diarrhea by amplicon sequencing
by: Haiyan Liu, et al.
Published: (2019-04-01)

High Throughput Sequencing for the Detection and Characterization of RNA Viruses
by: Amy H. Fitzpatrick, et al.
Published: (2021-02-01)

An Economical and Flexible Dual Barcoding, Two-Step PCR Approach for Highly Multiplexed Amplicon Sequencing
by: Petra Pjevac, et al.
Published: (2021-05-01)

Optimisation and Benchmarking of Targeted Amplicon Sequencing for Mycobiome Analysis of Respiratory Specimens
by: Nur A’tikah Binte Mohamed Ali, et al.
Published: (2019-10-01)

Whole lung lavage using a rapid infusion system to treat a patient with pulmonary alveolar proteinosis
by: Seung Won Ra, et al.
Published: (2020-01-01)

Culturing Bacteria From Fermentation Pit Muds of Baijiu With Culturomics and Amplicon-Based Metagenomic Approaches
by: Jialiang Xu, et al.
Published: (2020-06-01)

Autoimmune pulmonary alveolar proteinosis successfully treated with lung lavage in an adolescent patient: a case report
by: Abdalla Mohmed Alasiri, et al.
Published: (2021-07-01)