Etiology of Severe Pneumonia in Children in Alveolar Lavage Fluid Using a High-Throughput Gene Targeted Amplicon Sequencing Assay

Etiology of Severe Pneumonia in Children in Alveolar Lavage Fluid Using a High-Throughput Gene Targeted Amplicon Sequencing Assay

Objective: To evaluate the diagnostic value of a high-throughput gene targeted amplicon sequencing (TAS) assay for detecting pathogenic microorganisms in alveolar lavage fluid (ALF) from children with severe community-acquired pneumonia (SCAP).Methods: A retrospective study was performed on 48 froze...

Full description

Bibliographic Details
Main Authors: Fei Li, Yin Wang, Yuhan Zhang, Peng Shi, Linfeng Cao, LiYun Su, Qiguo Zhu, Libo Wang, Roujian Lu, Wenjie Tan, Jun Shen
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-06-01
Series:Frontiers in Pediatrics
Subjects:
high-throughput sequencing
targeted amplicon sequencing
multiplex polymerase chain reaction
severe community-acquired pneumonia
alveolar lavage fluid
children
Online Access:https://www.frontiersin.org/articles/10.3389/fped.2021.659164/full

Internet

https://www.frontiersin.org/articles/10.3389/fped.2021.659164/full

Similar Items