Efficient Overproduction of Active Nitrile Hydratase by Coupling Expression Induction and Enzyme Maturation via Programming a Controllable Cobalt-Responsive Gene Circuit

Efficient Overproduction of Active Nitrile Hydratase by Coupling Expression Induction and Enzyme Maturation via Programming a Controllable Cobalt-Responsive Gene Circuit

A robust and portable expression system is of great importance in enzyme production, metabolic engineering, and synthetic biology, which maximizes the performance of the engineered system. In this study, a tailor-made cobalt-induced expression system (CIES) was developed for low-cost and eco-friendl...

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Bibliographic Details
Main Authors: Laichuang Han, Wenjing Cui, Qiao Lin, Qiaoqing Chen, Feiya Suo, Ke Ma, Yang Wang, Wenliang Hao, Zhongyi Cheng, Zhemin Zhou
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-03-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
expression system
nitrile hydratase
cobalt-induced
metal homeostasis
protein expression
Online Access:https://www.frontiersin.org/article/10.3389/fbioe.2020.00193/full

Internet

https://www.frontiersin.org/article/10.3389/fbioe.2020.00193/full

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