A single centrifugation method for isolating fat droplets from cells and tissues

A single centrifugation method for isolating fat droplets from cells and tissues

Fat droplets (FDs) have important roles in cellular energy regulation. Isolating FDs from either cells or tissue continues to be important for studying these organelles. Here, we describe a procedure wherein whole homogenates of cultured cells or tissue are fractionated with a single centrifugation...

Full description

Bibliographic Details
Main Authors: Lydia-Ann L.S. Harris, Trevor M. Shew, James R. Skinner, Nathan E. Wolins
Format: Article
Language:English
Published: Elsevier 2012-05-01
Series:Journal of Lipid Research
Subjects:
adipose tissue
fatty acid/metabolism
lipid droplets
lipids
liver
brown adipose tissue
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520392336
id doaj-2ead55cdce644fa58516c0c560bf9b12
record_format Article
spelling doaj-2ead55cdce644fa58516c0c560bf9b122021-04-28T06:02:35ZengElsevierJournal of Lipid Research0022-22752012-05-0153510211025A single centrifugation method for isolating fat droplets from cells and tissuesLydia-Ann L.S. Harris0Trevor M. Shew1James R. Skinner2Nathan E. Wolins3Center for Human Nutrition, Washington University School of Medicine, St. Louis, MOCenter for Human Nutrition, Washington University School of Medicine, St. Louis, MOCenter for Human Nutrition, Washington University School of Medicine, St. Louis, MOTo whom correspondence should be addressed; Center for Human Nutrition, Washington University School of Medicine, St. Louis, MOFat droplets (FDs) have important roles in cellular energy regulation. Isolating FDs from either cells or tissue continues to be important for studying these organelles. Here, we describe a procedure wherein whole homogenates of cultured cells or tissue are fractionated with a single centrifugation step in a standard microcentrifuge. This procedure reproducibly yields three fractions highly enriched in either FDs, soluble cellular components, or sedimentable organelles/membranes.http://www.sciencedirect.com/science/article/pii/S0022227520392336adipose tissuefatty acid/metabolismlipid dropletslipidsliverbrown adipose tissue
collection DOAJ
language English
format Article
sources DOAJ
author Lydia-Ann L.S. Harris
Trevor M. Shew
James R. Skinner
Nathan E. Wolins
spellingShingle Lydia-Ann L.S. Harris
Trevor M. Shew
James R. Skinner
Nathan E. Wolins
A single centrifugation method for isolating fat droplets from cells and tissues
Journal of Lipid Research
adipose tissue
fatty acid/metabolism
lipid droplets
lipids
liver
brown adipose tissue
author_facet Lydia-Ann L.S. Harris
Trevor M. Shew
James R. Skinner
Nathan E. Wolins
author_sort Lydia-Ann L.S. Harris
title A single centrifugation method for isolating fat droplets from cells and tissues
title_short A single centrifugation method for isolating fat droplets from cells and tissues
title_full A single centrifugation method for isolating fat droplets from cells and tissues
title_fullStr A single centrifugation method for isolating fat droplets from cells and tissues
title_full_unstemmed A single centrifugation method for isolating fat droplets from cells and tissues
title_sort single centrifugation method for isolating fat droplets from cells and tissues
publisher Elsevier
series Journal of Lipid Research
issn 0022-2275
publishDate 2012-05-01
description Fat droplets (FDs) have important roles in cellular energy regulation. Isolating FDs from either cells or tissue continues to be important for studying these organelles. Here, we describe a procedure wherein whole homogenates of cultured cells or tissue are fractionated with a single centrifugation step in a standard microcentrifuge. This procedure reproducibly yields three fractions highly enriched in either FDs, soluble cellular components, or sedimentable organelles/membranes.
topic adipose tissue
fatty acid/metabolism
lipid droplets
lipids
liver
brown adipose tissue
url http://www.sciencedirect.com/science/article/pii/S0022227520392336
work_keys_str_mv AT lydiaannlsharris asinglecentrifugationmethodforisolatingfatdropletsfromcellsandtissues
AT trevormshew asinglecentrifugationmethodforisolatingfatdropletsfromcellsandtissues
AT jamesrskinner asinglecentrifugationmethodforisolatingfatdropletsfromcellsandtissues
AT nathanewolins asinglecentrifugationmethodforisolatingfatdropletsfromcellsandtissues
AT lydiaannlsharris singlecentrifugationmethodforisolatingfatdropletsfromcellsandtissues
AT trevormshew singlecentrifugationmethodforisolatingfatdropletsfromcellsandtissues
AT jamesrskinner singlecentrifugationmethodforisolatingfatdropletsfromcellsandtissues
AT nathanewolins singlecentrifugationmethodforisolatingfatdropletsfromcellsandtissues
_version_ 1721504423668088832

Similar Items