A single centrifugation method for isolating fat droplets from cells and tissues

Fat droplets (FDs) have important roles in cellular energy regulation. Isolating FDs from either cells or tissue continues to be important for studying these organelles. Here, we describe a procedure wherein whole homogenates of cultured cells or tissue are fractionated with a single centrifugation...

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Bibliographic Details
Main Authors: Lydia-Ann L.S. Harris, Trevor M. Shew, James R. Skinner, Nathan E. Wolins
Format: Article
Language:English
Published: Elsevier 2012-05-01
Series:Journal of Lipid Research
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520392336