A single centrifugation method for isolating fat droplets from cells and tissues

A single centrifugation method for isolating fat droplets from cells and tissues

Fat droplets (FDs) have important roles in cellular energy regulation. Isolating FDs from either cells or tissue continues to be important for studying these organelles. Here, we describe a procedure wherein whole homogenates of cultured cells or tissue are fractionated with a single centrifugation...

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Bibliographic Details
Main Authors: Lydia-Ann L.S. Harris, Trevor M. Shew, James R. Skinner, Nathan E. Wolins
Format: Article
Language:English
Published: Elsevier 2012-05-01
Series:Journal of Lipid Research
Subjects:
adipose tissue
fatty acid/metabolism
lipid droplets
lipids
liver
brown adipose tissue
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520392336
Description
Summary:Fat droplets (FDs) have important roles in cellular energy regulation. Isolating FDs from either cells or tissue continues to be important for studying these organelles. Here, we describe a procedure wherein whole homogenates of cultured cells or tissue are fractionated with a single centrifugation step in a standard microcentrifuge. This procedure reproducibly yields three fractions highly enriched in either FDs, soluble cellular components, or sedimentable organelles/membranes.
ISSN:0022-2275

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