An Analytically and Diagnostically Sensitive RNA Extraction and RT-qPCR Protocol for Peripheral Blood Mononuclear Cells

Reliable extraction and sensitive detection of RNA from human peripheral blood mononuclear cells (PBMCs) is critical for a broad spectrum of immunology research and clinical diagnostics. RNA analysis platforms are dependent upon high-quality and high-quantity RNA; however, sensitive detection of spe...

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Bibliographic Details
Main Authors: Daniel J. Browne, Jamie L. Brady, Ashley J. Waardenberg, Claire Loiseau, Denise L. Doolan
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-03-01
Series:Frontiers in Immunology
Subjects:
RNA
Online Access:https://www.frontiersin.org/article/10.3389/fimmu.2020.00402/full