Functional efficiency of PCR vectors in vitro and at the organism level.

The functional efficiency of the expression cassettes integrated into a plasmid and a PCR- amplified fragment was comparatively analyzed after transient transfection in vitro or introduction into the developing embryo of Danio rerio. The cassettes contained the reporter genes, luciferase of Photinus...

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Bibliographic Details
Main Authors: Dina R Safina, Polina I Selina, Marina P Roschina, Maria A Karaseva, Alexey A Komissarov, Ilya V Demidyuk, Eugene D Sverdlov, Sergey V Kostrov
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2020-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0232045