A bovine cell line that can be infected by natural sheep scrapie prions.

Cell culture systems represent a crucial part in basic prion research; yet, cell lines that are susceptible to prions, especially to field isolated prions that were not adapted to rodents, are very rare. The purpose of this study was to identify and characterize a cell line that was susceptible to r...

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Main Authors: Anja M Oelschlegel, Markus Geissen, Matthias Lenk, Roland Riebe, Marlies Angermann, Herman Schatzl, Martin H Groschup
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2015-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4286239?pdf=render
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spelling doaj-2c4a9d196765481db11e581a9a86325e2020-11-24T21:27:11ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01101e011715410.1371/journal.pone.0117154A bovine cell line that can be infected by natural sheep scrapie prions.Anja M OelschlegelMarkus GeissenMatthias LenkRoland RiebeMarlies AngermannHerman SchatzlMartin H GroschupCell culture systems represent a crucial part in basic prion research; yet, cell lines that are susceptible to prions, especially to field isolated prions that were not adapted to rodents, are very rare. The purpose of this study was to identify and characterize a cell line that was susceptible to ruminant-derived prions and to establish a stable prion infection within it. Based on species and tissue of origin as well as PrP expression rate, we pre-selected a total of 33 cell lines that were then challenged with natural and with mouse propagated BSE or scrapie inocula. Here, we report the successful infection of a non-transgenic bovine cell line, a sub-line of the bovine kidney cell line MDBK, with natural sheep scrapie prions. This cell line retained the scrapie infection for more than 200 passages. Selective cloning resulted in cell populations with increased accumulation of PrPres, although this treatment was not mandatory for retaining the infection. The infection remained stable, even under suboptimal culture conditions. The resulting infectivity of the cells was confirmed by mouse bioassay (Tgbov mice, Tgshp mice). We believe that PES cells used together with other prion permissive cell lines will prove a valuable tool for ongoing efforts to understand and defeat prions and prion diseases.http://europepmc.org/articles/PMC4286239?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Anja M Oelschlegel
Markus Geissen
Matthias Lenk
Roland Riebe
Marlies Angermann
Herman Schatzl
Martin H Groschup
spellingShingle Anja M Oelschlegel
Markus Geissen
Matthias Lenk
Roland Riebe
Marlies Angermann
Herman Schatzl
Martin H Groschup
A bovine cell line that can be infected by natural sheep scrapie prions.
PLoS ONE
author_facet Anja M Oelschlegel
Markus Geissen
Matthias Lenk
Roland Riebe
Marlies Angermann
Herman Schatzl
Martin H Groschup
author_sort Anja M Oelschlegel
title A bovine cell line that can be infected by natural sheep scrapie prions.
title_short A bovine cell line that can be infected by natural sheep scrapie prions.
title_full A bovine cell line that can be infected by natural sheep scrapie prions.
title_fullStr A bovine cell line that can be infected by natural sheep scrapie prions.
title_full_unstemmed A bovine cell line that can be infected by natural sheep scrapie prions.
title_sort bovine cell line that can be infected by natural sheep scrapie prions.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2015-01-01
description Cell culture systems represent a crucial part in basic prion research; yet, cell lines that are susceptible to prions, especially to field isolated prions that were not adapted to rodents, are very rare. The purpose of this study was to identify and characterize a cell line that was susceptible to ruminant-derived prions and to establish a stable prion infection within it. Based on species and tissue of origin as well as PrP expression rate, we pre-selected a total of 33 cell lines that were then challenged with natural and with mouse propagated BSE or scrapie inocula. Here, we report the successful infection of a non-transgenic bovine cell line, a sub-line of the bovine kidney cell line MDBK, with natural sheep scrapie prions. This cell line retained the scrapie infection for more than 200 passages. Selective cloning resulted in cell populations with increased accumulation of PrPres, although this treatment was not mandatory for retaining the infection. The infection remained stable, even under suboptimal culture conditions. The resulting infectivity of the cells was confirmed by mouse bioassay (Tgbov mice, Tgshp mice). We believe that PES cells used together with other prion permissive cell lines will prove a valuable tool for ongoing efforts to understand and defeat prions and prion diseases.
url http://europepmc.org/articles/PMC4286239?pdf=render
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