Fluorimetric determination of sphingosine and its application to natural mixtures of glycosphingolipids
A sensitive estimation of sphingosine, by measurement of the fluorescence of a complex formed with 1-naphthylamino-4-sulfonic acid, is described. The practical range is 5-35 nmoles sphingosine. The method is used to estimate, in terms of sphingosine, amounts of ceramide and glycosphingolipids. The i...
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| Format: | Article |
| Language: | English |
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Elsevier
1970-03-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520430081 |
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doaj-29d4488341b444de87709f80141350062021-04-24T05:55:02ZengElsevierJournal of Lipid Research0022-22751970-03-01112164166Fluorimetric determination of sphingosine and its application to natural mixtures of glycosphingolipidsL. ColesG.M. GrayA sensitive estimation of sphingosine, by measurement of the fluorescence of a complex formed with 1-naphthylamino-4-sulfonic acid, is described. The practical range is 5-35 nmoles sphingosine. The method is used to estimate, in terms of sphingosine, amounts of ceramide and glycosphingolipids. The isolation of microamounts (5-30 micro g) of individual glycosphingolipids from a mixture, and their quantitative estimation is described. The percentage composition of a glycosphingolipid mixture from the kidneys of adult C57/BL male mice is given.http://www.sciencedirect.com/science/article/pii/S0022227520430081 |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
L. Coles G.M. Gray |
| spellingShingle |
L. Coles G.M. Gray Fluorimetric determination of sphingosine and its application to natural mixtures of glycosphingolipids Journal of Lipid Research |
| author_facet |
L. Coles G.M. Gray |
| author_sort |
L. Coles |
| title |
Fluorimetric determination of sphingosine and its application to natural mixtures of glycosphingolipids |
| title_short |
Fluorimetric determination of sphingosine and its application to natural mixtures of glycosphingolipids |
| title_full |
Fluorimetric determination of sphingosine and its application to natural mixtures of glycosphingolipids |
| title_fullStr |
Fluorimetric determination of sphingosine and its application to natural mixtures of glycosphingolipids |
| title_full_unstemmed |
Fluorimetric determination of sphingosine and its application to natural mixtures of glycosphingolipids |
| title_sort |
fluorimetric determination of sphingosine and its application to natural mixtures of glycosphingolipids |
| publisher |
Elsevier |
| series |
Journal of Lipid Research |
| issn |
0022-2275 |
| publishDate |
1970-03-01 |
| description |
A sensitive estimation of sphingosine, by measurement of the fluorescence of a complex formed with 1-naphthylamino-4-sulfonic acid, is described. The practical range is 5-35 nmoles sphingosine. The method is used to estimate, in terms of sphingosine, amounts of ceramide and glycosphingolipids. The isolation of microamounts (5-30 micro g) of individual glycosphingolipids from a mixture, and their quantitative estimation is described. The percentage composition of a glycosphingolipid mixture from the kidneys of adult C57/BL male mice is given. |
| url |
http://www.sciencedirect.com/science/article/pii/S0022227520430081 |
| work_keys_str_mv |
AT lcoles fluorimetricdeterminationofsphingosineanditsapplicationtonaturalmixturesofglycosphingolipids AT gmgray fluorimetricdeterminationofsphingosineanditsapplicationtonaturalmixturesofglycosphingolipids |
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1721511302680018944 |