Fluorimetric determination of sphingosine and its application to natural mixtures of glycosphingolipids
A sensitive estimation of sphingosine, by measurement of the fluorescence of a complex formed with 1-naphthylamino-4-sulfonic acid, is described. The practical range is 5-35 nmoles sphingosine. The method is used to estimate, in terms of sphingosine, amounts of ceramide and glycosphingolipids. The i...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1970-03-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520430081 |