Quantitative Multiplex Immunohistochemistry Reveals Myeloid-Inflamed Tumor-Immune Complexity Associated with Poor Prognosis

Quantitative Multiplex Immunohistochemistry Reveals Myeloid-Inflamed Tumor-Immune Complexity Associated with Poor Prognosis

Here, we describe a multiplexed immunohistochemical platform with computational image processing workflows, including image cytometry, enabling simultaneous evaluation of 12 biomarkers in one formalin-fixed paraffin-embedded tissue section. To validate this platform, we used tissue microarrays conta...

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Bibliographic Details
Main Authors: Takahiro Tsujikawa, Sushil Kumar, Rohan N. Borkar, Vahid Azimi, Guillaume Thibault, Young Hwan Chang, Ariel Balter, Rie Kawashima, Gina Choe, David Sauer, Edward El Rassi, Daniel R. Clayburgh, Molly F. Kulesz-Martin, Eric R. Lutz, Lei Zheng, Elizabeth M. Jaffee, Patrick Leyshock, Adam A. Margolin, Motomi Mori, Joe W. Gray, Paul W. Flint, Lisa M. Coussens
Format: Article
Language:English
Published: Elsevier 2017-04-01
Series:Cell Reports
Subjects:
cancer immunology
multiplex
immunohistochemistry
digital pathology
image cytometry
head and neck cancer
pancreatic cancer
tissue biomarker
Online Access:http://www.sciencedirect.com/science/article/pii/S2211124717303832

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http://www.sciencedirect.com/science/article/pii/S2211124717303832

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