Quantifying accuracy and heterogeneity in single-molecule super-resolution microscopy
Standard benchmarking of single-molecule localization microscopy cannot quantify nanoscale accuracy of arbitrary datasets. Here, the authors present Wasserstein-induced flux, a method using a chosen perturbation and knowledge of the imaging system to measure confidence of individual localizations.
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
Nature Publishing Group
2020-12-01
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Series: | Nature Communications |
Online Access: | https://doi.org/10.1038/s41467-020-20056-9 |