Quantifying accuracy and heterogeneity in single-molecule super-resolution microscopy

Standard benchmarking of single-molecule localization microscopy cannot quantify nanoscale accuracy of arbitrary datasets. Here, the authors present Wasserstein-induced flux, a method using a chosen perturbation and knowledge of the imaging system to measure confidence of individual localizations.

Bibliographic Details
Main Authors: Hesam Mazidi, Tianben Ding, Arye Nehorai, Matthew D. Lew
Format: Article
Language:English
Published: Nature Publishing Group 2020-12-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-020-20056-9