Optimization of PCR amplification of maize microsatellite loci

Maize (Zea mays L.) microsatellite loci are useful as genetic markers because they are numerous, occur in every chromosome, and have a high content of polymorphism information. Furthermore, they can be amplified by PCR and the resulting fragments resolved on agarose gels. A major problem, however, i...

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Bibliographic Details
Main Authors: Ogliari Juliana Bernardi, Boscariol Raquel L., Camargo Luis E.A.
Format: Article
Language:English
Published: Sociedade Brasileira de Genética 2000-01-01
Series:Genetics and Molecular Biology
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200026