Preparing unbiased T cell receptor and antibody cDNA libraries for the deep next generation sequencing profiling

Preparing unbiased T cell receptor and antibody cDNA libraries for the deep next generation sequencing profiling

High-throughput sequencing has the power to reveal the nature of adaptive immunity as represented by the full complexity of T cell receptor (TCR) and antibody (IG) repertoires, but is at present severely compromised by the quantitative bias, bottlenecks, and accumulated errors that inevitably occur...

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Bibliographic Details
Main Authors: Ilgar Z Mamedov, Olga V Britanova, Ivan V Zvyagin, Maria A Turchaninova, Dmitriy A Bolotin, Ekaterina V Putintseva, Yuriy B Lebedev, Dmitriy M Chudakov
Format: Article
Language:English
Published: Frontiers Media S.A. 2013-12-01
Series:Frontiers in Immunology
Subjects:
T cell receptor (TCR)
NGS applications
T cell receptor repertoir
TCR repertoires
BCR repertoires
cDNA libraries
Online Access:http://journal.frontiersin.org/Journal/10.3389/fimmu.2013.00456/full

Internet

http://journal.frontiersin.org/Journal/10.3389/fimmu.2013.00456/full

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