In vitro anthelmintic activity of grape pomace extract against gastrointestinal nematodes of naturally infected sheep

• Main Authors: Suelen Carolina Silva Soares, Gilmar Cotrin de Lima, Antonio Carlos Laurentiz, Aline Féboli, Luciano Alves dos Anjos, Matheus Souza de Paula Carlis, Rosimeire da Silva Filardi, Rosangela da Silva de Laurentiz
• Format: Article
• Language: English
• Published: Taylor & Francis Group 2018-12-01
• Series: International Journal of Veterinary Science and Medicine
• Online Access: http://www.sciencedirect.com/science/article/pii/S2314459918301534

Grape pomace obtained as a byproduct of industrial processing of grapes retains nutrients and substances with anthelmintic potential such as saponins, tannins, and flavonoids. Therefore, this study evaluated the in vitro ovicidal and larvicidal activity of hydroalcoholic grape pomace extract against gastrointestinal nematodes of sheep. The anthelmintic evaluation was performed by in vitro assays with eggs and larvae of nematodes obtained from naturally infected donor sheep. The grape pomace extract showed high in vitro ovicidal and larvicidal activity with LD50 values of 0.30 mg/mL for egg hatching inhibition, 1.01 mg/mL for larval development inhibition and 100% efficacy in larval migration inhibition assays at all concentrations evaluated. The effect of tannins was evaluated by the addition of 50 mg/mL polyvinyl polypyrrolidone to grape pomace extract at the concentration of 12.5 mg/mL. The in vitro ovicidal activity of grape pomace was reduced by only 15% after polyvinyl polypyrrolidone addition, indicating that other phytochemicals also contribute to the ovicidal activity displayed by the extract. Our results demonstrate that grape pomace exhibits in vitro anthelmintic activity, suggesting that, beyond its nutritional potential, this pomace can also be an ally for gastrointestinal nematode control in sheep. Keywords: Gastrointestinal nematodes, Haemonchus contortus, Ovicidal and larvicidal activities, Phytochemical, Tannin