CryoEM structure of the antibacterial target PBP1b at 3.3 Å resolution

Our structural understanding of class A penicillin binding proteins is incomplete due to the difficulty in their crystallization and the complexity of their substrates. Here, authors determine the structure of the 83 kDa class A PBP from Escherichia coli, PBP1b, using cryogenic electron microscopy a...

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Main Authors:	Nathanael A. Caveney, Sean D. Workman, Rui Yan, Claire E. Atkinson, Zhiheng Yu, Natalie C. J. Strynadka
Format:	Article
Language:	English
Published:	Nature Publishing Group 2021-05-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-021-23063-6

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spelling	doaj-26f4e40c535f443f997600b2ad246b012021-05-16T11:10:46ZengNature Publishing GroupNature Communications2041-17232021-05-011211710.1038/s41467-021-23063-6CryoEM structure of the antibacterial target PBP1b at 3.3 Å resolutionNathanael A. Caveney0Sean D. Workman1Rui Yan2Claire E. Atkinson3Zhiheng Yu4Natalie C. J. Strynadka5Department of Biochemistry and Molecular Biology, University of British ColumbiaDepartment of Biochemistry and Molecular Biology, University of British ColumbiaCryoEM Shared Resources, Howard Hughes Medical InstituteDepartment of Biochemistry and Molecular Biology, University of British ColumbiaCryoEM Shared Resources, Howard Hughes Medical InstituteDepartment of Biochemistry and Molecular Biology, University of British ColumbiaOur structural understanding of class A penicillin binding proteins is incomplete due to the difficulty in their crystallization and the complexity of their substrates. Here, authors determine the structure of the 83 kDa class A PBP from Escherichia coli, PBP1b, using cryogenic electron microscopy and a styrene maleic acid anhydride membrane mimetic.https://doi.org/10.1038/s41467-021-23063-6
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Nathanael A. Caveney Sean D. Workman Rui Yan Claire E. Atkinson Zhiheng Yu Natalie C. J. Strynadka
spellingShingle	Nathanael A. Caveney Sean D. Workman Rui Yan Claire E. Atkinson Zhiheng Yu Natalie C. J. Strynadka CryoEM structure of the antibacterial target PBP1b at 3.3 Å resolution Nature Communications
author_facet	Nathanael A. Caveney Sean D. Workman Rui Yan Claire E. Atkinson Zhiheng Yu Natalie C. J. Strynadka
author_sort	Nathanael A. Caveney
title	CryoEM structure of the antibacterial target PBP1b at 3.3 Å resolution
title_short	CryoEM structure of the antibacterial target PBP1b at 3.3 Å resolution
title_full	CryoEM structure of the antibacterial target PBP1b at 3.3 Å resolution
title_fullStr	CryoEM structure of the antibacterial target PBP1b at 3.3 Å resolution
title_full_unstemmed	CryoEM structure of the antibacterial target PBP1b at 3.3 Å resolution
title_sort	cryoem structure of the antibacterial target pbp1b at 3.3 å resolution
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2021-05-01
description	Our structural understanding of class A penicillin binding proteins is incomplete due to the difficulty in their crystallization and the complexity of their substrates. Here, authors determine the structure of the 83 kDa class A PBP from Escherichia coli, PBP1b, using cryogenic electron microscopy and a styrene maleic acid anhydride membrane mimetic.
url	https://doi.org/10.1038/s41467-021-23063-6
work_keys_str_mv	AT nathanaelacaveney cryoemstructureoftheantibacterialtargetpbp1bat33aresolution AT seandworkman cryoemstructureoftheantibacterialtargetpbp1bat33aresolution AT ruiyan cryoemstructureoftheantibacterialtargetpbp1bat33aresolution AT claireeatkinson cryoemstructureoftheantibacterialtargetpbp1bat33aresolution AT zhihengyu cryoemstructureoftheantibacterialtargetpbp1bat33aresolution AT nataliecjstrynadka cryoemstructureoftheantibacterialtargetpbp1bat33aresolution
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CryoEM structure of the antibacterial target PBP1b at 3.3 Å resolution

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