Isothermal amplification and rapid detection of Klebsiella pneumoniae based on the multiple cross displacement amplification (MCDA) and gold nanoparticle lateral flow biosensor (LFB).

Klebsiella pneumoniae (K. pneumoniae) is a frequent pathogen causing nosocomial infections and outbreaks. We developed a multiple cross displacement amplification (MCDA) assay for the detection of K. pneumoniae, which can get the positive results within 40 minutes' isothermal amplification. Gol...

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Bibliographic Details
Main Authors: Lina Niu, Fan Zhao, Jinlong Chen, Jinqing Nong, Chunmei Wang, Jing Wang, Naishu Gao, Xiaoxue Zhu, Lei Wu, Shoukui Hu
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2018-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC6166938?pdf=render