Fluorolabeling of the PPTase-Related Chemical Tags: Comparative Study of Different Membrane Receptors and Different Fluorophores in the Labeling Reactions

Fluorolabeling of the PPTase-Related Chemical Tags: Comparative Study of Different Membrane Receptors and Different Fluorophores in the Labeling Reactions

The set-up of an advanced imaging experiment requires a careful selection of suitable labeling strategies and fluorophores for the tagging of the molecules of interest. Here we provide an experimental workflow to allow evaluation of fluorolabeling performance of the chemical tags target of phosphopa...

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Bibliographic Details
Main Authors: Rosy Amodeo, Domenica Convertino, Mariantonietta Calvello, Lorenzo Ceccarelli, Fulvio Bonsignore, Cosetta Ravelli, Antonino Cattaneo, Claudia Martini, Stefano Luin, Stefania Mitola, Giovanni Signore, Laura Marchetti
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-08-01
Series:Frontiers in Molecular Biosciences
Subjects:
fluorophore
Single molecule labeling
PPTase labeling
Fluorescent Nerve Growth Factor
chemical tag
surface receptor
Online Access:https://www.frontiersin.org/article/10.3389/fmolb.2020.00195/full

Internet

https://www.frontiersin.org/article/10.3389/fmolb.2020.00195/full

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