Differentiation of human pluripotent stem cells into functional airway basal stem cells

Differentiation of human pluripotent stem cells into functional airway basal stem cells

Summary: Airway basal cells play an essential role in the maintenance of the airway epithelium. Here, we provide a detailed directed differentiation protocol to generate ‘‘induced basal cells (iBCs)’’ from human pluripotent stem cells. iBCs recapitulate biological and functional properties of airway...

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Bibliographic Details
Main Authors: Shingo Suzuki, Finn J. Hawkins, Cristina Barillà, Mary Lou Beermann, Darrell N. Kotton, Brian R. Davis
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell culture
Cell isolation
Flow Cytometry/Mass Cytometry
Stem Cells
Cell Differentiation
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721003907

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http://www.sciencedirect.com/science/article/pii/S2666166721003907

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