A Three-Way Combinatorial CRISPR Screen for Analyzing Interactions among Druggable Targets

A Three-Way Combinatorial CRISPR Screen for Analyzing Interactions among Druggable Targets

Summary: We present a CRISPR-based multi-gene knockout screening system and toolkits for extensible assembly of barcoded high-order combinatorial guide RNA libraries en masse. We apply this system for systematically identifying not only pairwise but also three-way synergistic therapeutic target comb...

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Bibliographic Details
Main Authors: Peng Zhou, Becky K.C. Chan, Yuk Kei Wan, Chaya T.L. Yuen, Gigi C.G. Choi, Xinran Li, Cindy S.W. Tong, Sophia S.W. Zhong, Jieran Sun, Yufan Bao, Silvia Y.L. Mak, Maggie Z.Y. Chow, Jien Vei Khaw, Suet Yi Leung, Zongli Zheng, Lydia W.T. Cheung, Kaeling Tan, Koon Ho Wong, H.Y. Edwin Chan, Alan S.L. Wong
Format: Article
Language:English
Published: Elsevier 2020-08-01
Series:Cell Reports
Subjects:
combinatorial genetics
CombiGEM
CRISPR
combination therapy
high-throughput screening
cancer
Online Access:http://www.sciencedirect.com/science/article/pii/S2211124720310056

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http://www.sciencedirect.com/science/article/pii/S2211124720310056

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