Electroporation and microinjection successfully deliver single-stranded and duplex DNA into live cells as detected by FRET measurements.

Electroporation and microinjection successfully deliver single-stranded and duplex DNA into live cells as detected by FRET measurements.

Förster resonance energy transfer (FRET) technology relies on the close proximity of two compatible fluorophores for energy transfer. Tagged (Cy3 and Cy5) complementary DNA strands forming a stable duplex and a doubly-tagged single strand were shown to demonstrate FRET outside of a cellular environm...

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Bibliographic Details
Main Authors: Rosemary A Bamford, Zheng-yun Zhao, Neil A Hotchin, Iain B Styles, Gerard B Nash, James H R Tucker, Roy Bicknell
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3995676?pdf=render

Internet

http://europepmc.org/articles/PMC3995676?pdf=render

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