Classification of pmoA amplicon pyrosequences using BLAST and the lowest common ancestor method in MEGAN

Classification of pmoA amplicon pyrosequences using BLAST and the lowest common ancestor method in MEGAN

The classification of high-throughput sequencing data of protein-encoding genes is not as well established as for 16S rRNA. The objective of this work was to develop a simple and accurate method of classifying large datasets of pmoA sequences, a common marker for methanotrophic bacteria. A taxonomic...

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Bibliographic Details
Main Authors: Marc Gregory Dumont, Claudia eLüke, Yongcui eDeng, Peter eFrenzel
Format: Article
Language:English
Published: Frontiers Media S.A. 2014-02-01
Series:Frontiers in Microbiology
Subjects:
pyrosequencing
diversity
methanotroph
pmoA
NGS data analysis
Online Access:http://journal.frontiersin.org/Journal/10.3389/fmicb.2014.00034/full

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