Classification of pmoA amplicon pyrosequences using BLAST and the lowest common ancestor method in MEGAN

The classification of high-throughput sequencing data of protein-encoding genes is not as well established as for 16S rRNA. The objective of this work was to develop a simple and accurate method of classifying large datasets of pmoA sequences, a common marker for methanotrophic bacteria. A taxonomic...

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Bibliographic Details
Main Authors: Marc Gregory Dumont, Claudia eLüke, Yongcui eDeng, Peter eFrenzel
Format: Article
Language:English
Published: Frontiers Media S.A. 2014-02-01
Series:Frontiers in Microbiology
Subjects:
Online Access:http://journal.frontiersin.org/Journal/10.3389/fmicb.2014.00034/full