High-throughput in vivo mapping of RNA accessible interfaces to identify functional sRNA binding sites

Mapping RNA accessibility is valuable for identifying functional/regulatory RNA regions. Here the authors introduce INTERFACE, an intracellular method that quantifies antisense hybridization efficacy of any number of RNA regions simultaneously via a transcriptional elongation output, measurable via...

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Main Authors:	Mia K. Mihailovic, Jorge Vazquez-Anderson, Yan Li, Victoria Fry, Praveen Vimalathas, Daniel Herrera, Richard A. Lease, Warren B. Powell, Lydia M. Contreras
Format:	Article
Language:	English
Published:	Nature Publishing Group 2018-10-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-018-06207-z

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spelling	doaj-23489d4076474f688e376a0c9e4c75782021-05-11T10:23:35ZengNature Publishing GroupNature Communications2041-17232018-10-019111610.1038/s41467-018-06207-zHigh-throughput in vivo mapping of RNA accessible interfaces to identify functional sRNA binding sitesMia K. Mihailovic0Jorge Vazquez-Anderson1Yan Li2Victoria Fry3Praveen Vimalathas4Daniel Herrera5Richard A. Lease6Warren B. Powell7Lydia M. Contreras8McKetta Department of Chemical Engineering, University of Texas at AustinMcKetta Department of Chemical Engineering, University of Texas at AustinDepartment of Operations Research and Financial Engineering, Princeton UniversityMcKetta Department of Chemical Engineering, University of Texas at AustinMcKetta Department of Chemical Engineering, University of Texas at AustinDepartment of Computer Science, University of Texas at AustinDepartment of Chemical and Biomolecular Engineering, The Ohio State UniversityDepartment of Operations Research and Financial Engineering, Princeton UniversityMcKetta Department of Chemical Engineering, University of Texas at AustinMapping RNA accessibility is valuable for identifying functional/regulatory RNA regions. Here the authors introduce INTERFACE, an intracellular method that quantifies antisense hybridization efficacy of any number of RNA regions simultaneously via a transcriptional elongation output, measurable via RNA-seqhttps://doi.org/10.1038/s41467-018-06207-z
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Mia K. Mihailovic Jorge Vazquez-Anderson Yan Li Victoria Fry Praveen Vimalathas Daniel Herrera Richard A. Lease Warren B. Powell Lydia M. Contreras
spellingShingle	Mia K. Mihailovic Jorge Vazquez-Anderson Yan Li Victoria Fry Praveen Vimalathas Daniel Herrera Richard A. Lease Warren B. Powell Lydia M. Contreras High-throughput in vivo mapping of RNA accessible interfaces to identify functional sRNA binding sites Nature Communications
author_facet	Mia K. Mihailovic Jorge Vazquez-Anderson Yan Li Victoria Fry Praveen Vimalathas Daniel Herrera Richard A. Lease Warren B. Powell Lydia M. Contreras
author_sort	Mia K. Mihailovic
title	High-throughput in vivo mapping of RNA accessible interfaces to identify functional sRNA binding sites
title_short	High-throughput in vivo mapping of RNA accessible interfaces to identify functional sRNA binding sites
title_full	High-throughput in vivo mapping of RNA accessible interfaces to identify functional sRNA binding sites
title_fullStr	High-throughput in vivo mapping of RNA accessible interfaces to identify functional sRNA binding sites
title_full_unstemmed	High-throughput in vivo mapping of RNA accessible interfaces to identify functional sRNA binding sites
title_sort	high-throughput in vivo mapping of rna accessible interfaces to identify functional srna binding sites
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2018-10-01
description	Mapping RNA accessibility is valuable for identifying functional/regulatory RNA regions. Here the authors introduce INTERFACE, an intracellular method that quantifies antisense hybridization efficacy of any number of RNA regions simultaneously via a transcriptional elongation output, measurable via RNA-seq
url	https://doi.org/10.1038/s41467-018-06207-z
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High-throughput in vivo mapping of RNA accessible interfaces to identify functional sRNA binding sites

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