Standard protocol for the PIGRET assay, a high-throughput reticulocyte Pig-a assay with an immunomagnetic separation, used in the interlaboratory trial organized by the Mammalian Mutagenicity Study Group of the Japanese Environmental Mutagen and Genome Society

Standard protocol for the PIGRET assay, a high-throughput reticulocyte Pig-a assay with an immunomagnetic separation, used in the interlaboratory trial organized by the Mammalian Mutagenicity Study Group of the Japanese Environmental Mutagen and Genome Society

Abstract The PIGRET assay is one of the Pig-a assays targeting reticulocytes (RETs), an in vivo genotoxicity evaluation method using flow cytometry with endogenous reporter glycosylphosphatidylinositol anchor protein. The PIGRET assay with RETs selectively enriched with anti-CD71 antibodies has seve...

Full description

Bibliographic Details
Main Authors: Satsuki Chikura, Takafumi Kimoto, Satoru Itoh, Hisakazu Sanada, Shigeharu Muto, Katsuyoshi Horibata
Format: Article
Language:English
Published: BMC 2021-03-01
Series:Genes and Environment
Subjects:
Pig-a assay
Glycosylphosphatidylinositol
Flow cytometry
Reticulocytes
In vivo gene mutation
CD59
Online Access:https://doi.org/10.1186/s41021-021-00181-7

Internet

https://doi.org/10.1186/s41021-021-00181-7

Similar Items