gscreend: modelling asymmetric count ratios in CRISPR screens to decrease experiment size and improve phenotype detection

gscreend: modelling asymmetric count ratios in CRISPR screens to decrease experiment size and improve phenotype detection

Abstract Pooled CRISPR screens are a powerful tool to probe genotype-phenotype relationships at genome-wide scale. However, criteria for optimal design are missing, and it remains unclear how experimental parameters affect results. Here, we report that random decreases in gRNA abundance are more lik...

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Bibliographic Details
Main Authors: Katharina Imkeller, Giulia Ambrosi, Michael Boutros, Wolfgang Huber
Format: Article
Language:English
Published: BMC 2020-03-01
Series:Genome Biology
Subjects:
CRISPR screen
Genetic perturbation screen
Experimental design
Biology-based model
Generative probabilistic model
gscreend package
Online Access:http://link.springer.com/article/10.1186/s13059-020-1939-1

Internet

http://link.springer.com/article/10.1186/s13059-020-1939-1

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