Highly Sensitive Bacteria Quantification Using Immunomagnetic Separation and Electrochemical Detection of Guanine-Labeled Secondary Beads

Highly Sensitive Bacteria Quantification Using Immunomagnetic Separation and Electrochemical Detection of Guanine-Labeled Secondary Beads

In this paper, we report the ultra-sensitive indirect electrochemical detection of E. coli O157:H7 using antibody functionalized primary (magnetic) beads for capture and polyguanine (polyG) oligonucleotide functionalized secondary (polystyrene) beads as an electrochemical tag. Vacuum filtration in c...

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Bibliographic Details
Main Authors: Harikrishnan Jayamohan, Bruce K. Gale, Bj Minson, Christopher J. Lambert, Neil Gordon, Himanshu J. Sant
Format: Article
Language:English
Published: MDPI AG 2015-05-01
Series:Sensors
Subjects:
Escherichia coli O157:H7 detection
biosensors
pathogen detection
electrochemical detection
differential pulse voltammetry
immunomagnetic separation
Online Access:http://www.mdpi.com/1424-8220/15/5/12034

Internet

http://www.mdpi.com/1424-8220/15/5/12034

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