Short hairpin RNA targeting of fibroblast activation protein inhibits tumor growth and improves the tumor microenvironment in a mouse model

Short hairpin RNA targeting of fibroblast activation protein inhibits tumor growth and improves the tumor microenvironment in a mouse model

Fibroblast activation protein (FAP) is a specific serine proteaseexpressed in tumor stroma proven to be a stimulatory factor inthe progression of some cancers. The purpose of this studywas to investigate the effects of FAP knockdown on tumorgrowth and the tumor microenvironment. Mice bearing 4T1subc...

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Main Authors: Fan Cai, Zhiyong Li, Chunting Wang, Shuang Xian, Guangchao Xu, Feng Peng, Yuquan Wei, You Lu
Format: Article
Language:English
Published: Korean Society for Biochemistry and Molecular Biology 2013-05-01
Series:BMB Reports
Subjects:
Angiogenesis
Breast cancer
Fibroblast activation protein
RNA interference
Tumor microenvironment
Online Access:http://bmbreports.org/jbmb/pdf.php?data=MTMwOTI3MTFAcGRmX3JhaW50cmFjZV9sZWV5c0AlNUI0Ni01JTVEMTMwNTI5MjIyNl8lMjgyNTItMjU3JTI5Qk1CXzEyLTE3Mi5wZGY=
id doaj-211947959bf74e75a971cea80704adad
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spelling doaj-211947959bf74e75a971cea80704adad2020-11-24T23:09:16ZengKorean Society for Biochemistry and Molecular BiologyBMB Reports1976-66961976-670X2013-05-01465252257http://dx.doi.org/10.5483/BMBRep.2013.46.5.172Short hairpin RNA targeting of fibroblast activation protein inhibits tumor growth and improves the tumor microenvironment in a mouse modelFan CaiZhiyong LiChunting WangShuang XianGuangchao XuFeng PengYuquan WeiYou LuFibroblast activation protein (FAP) is a specific serine proteaseexpressed in tumor stroma proven to be a stimulatory factor inthe progression of some cancers. The purpose of this studywas to investigate the effects of FAP knockdown on tumorgrowth and the tumor microenvironment. Mice bearing 4T1subcutaneous tumors were treated with liposome-shRNAcomplexes targeting FAP. Tumor volumes and weights weremonitored, and FAP, collagen, microvessel density (MVD),and apoptosis were measured. Our studies showed thatshRNA targeting of FAP in murine breast cancer reduces FAPexpression, inhibits tumor growth, promotes collagenaccumulation (38%), and suppresses angiogenesis (71.7%), aswell as promoting apoptosis (by threefold). We suggest thatFAP plays a role in tumor growth and in altering the tumormicroenvironment. Targeting FAP may therefore represent asupplementary therapy for breast cancer. [BMB Reports 2013;46(5): 252-257]http://bmbreports.org/jbmb/pdf.php?data=MTMwOTI3MTFAcGRmX3JhaW50cmFjZV9sZWV5c0AlNUI0Ni01JTVEMTMwNTI5MjIyNl8lMjgyNTItMjU3JTI5Qk1CXzEyLTE3Mi5wZGY=AngiogenesisBreast cancerFibroblast activation proteinRNA interferenceTumor microenvironment
collection DOAJ
language English
format Article
sources DOAJ
author Fan Cai
Zhiyong Li
Chunting Wang
Shuang Xian
Guangchao Xu
Feng Peng
Yuquan Wei
You Lu
spellingShingle Fan Cai
Zhiyong Li
Chunting Wang
Shuang Xian
Guangchao Xu
Feng Peng
Yuquan Wei
You Lu
Short hairpin RNA targeting of fibroblast activation protein inhibits tumor growth and improves the tumor microenvironment in a mouse model
BMB Reports
Angiogenesis
Breast cancer
Fibroblast activation protein
RNA interference
Tumor microenvironment
author_facet Fan Cai
Zhiyong Li
Chunting Wang
Shuang Xian
Guangchao Xu
Feng Peng
Yuquan Wei
You Lu
author_sort Fan Cai
title Short hairpin RNA targeting of fibroblast activation protein inhibits tumor growth and improves the tumor microenvironment in a mouse model
title_short Short hairpin RNA targeting of fibroblast activation protein inhibits tumor growth and improves the tumor microenvironment in a mouse model
title_full Short hairpin RNA targeting of fibroblast activation protein inhibits tumor growth and improves the tumor microenvironment in a mouse model
title_fullStr Short hairpin RNA targeting of fibroblast activation protein inhibits tumor growth and improves the tumor microenvironment in a mouse model
title_full_unstemmed Short hairpin RNA targeting of fibroblast activation protein inhibits tumor growth and improves the tumor microenvironment in a mouse model
title_sort short hairpin rna targeting of fibroblast activation protein inhibits tumor growth and improves the tumor microenvironment in a mouse model
publisher Korean Society for Biochemistry and Molecular Biology
series BMB Reports
issn 1976-6696
1976-670X
publishDate 2013-05-01
description Fibroblast activation protein (FAP) is a specific serine proteaseexpressed in tumor stroma proven to be a stimulatory factor inthe progression of some cancers. The purpose of this studywas to investigate the effects of FAP knockdown on tumorgrowth and the tumor microenvironment. Mice bearing 4T1subcutaneous tumors were treated with liposome-shRNAcomplexes targeting FAP. Tumor volumes and weights weremonitored, and FAP, collagen, microvessel density (MVD),and apoptosis were measured. Our studies showed thatshRNA targeting of FAP in murine breast cancer reduces FAPexpression, inhibits tumor growth, promotes collagenaccumulation (38%), and suppresses angiogenesis (71.7%), aswell as promoting apoptosis (by threefold). We suggest thatFAP plays a role in tumor growth and in altering the tumormicroenvironment. Targeting FAP may therefore represent asupplementary therapy for breast cancer. [BMB Reports 2013;46(5): 252-257]
topic Angiogenesis
Breast cancer
Fibroblast activation protein
RNA interference
Tumor microenvironment
url http://bmbreports.org/jbmb/pdf.php?data=MTMwOTI3MTFAcGRmX3JhaW50cmFjZV9sZWV5c0AlNUI0Ni01JTVEMTMwNTI5MjIyNl8lMjgyNTItMjU3JTI5Qk1CXzEyLTE3Mi5wZGY=
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