Lgr5 maintains stemness and regulates cell property in nasopharyngeal carcinoma through Wnt/β-catenin signaling pathway

Nasopharyngeal carcinoma (NPC) is a common malignant tumor in Southern China and Southeast Asia. In this study, we found that Leucine rich repeat containing G protein-coupled receptor 5 (Lgr5) was highly expressed in NPC tissues and marked NPC stem cells. Lgr5high tumors showed differential transcri...

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Main Authors:	Fangqi Li, Xiaole Song, Xuewen Li, Xiujuan Zhang, Xiaoyu Feng, Li Wang, Lun Xu, Jiqin Luo, Bijun Zhu, Wenwen Ren, Hongmeng Yu, Yiqun Yu
Format:	Article
Language:	English
Published:	Elsevier 2020-08-01
Series:	Stem Cell Research
Subjects:	Lgr5 Nasopharyngeal carcinoma Stem cells Epithelial-mesenchymal transition Wnt/β-catenin pathway
Online Access:	http://www.sciencedirect.com/science/article/pii/S1873506120302178

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spelling	doaj-1fdc9c670cee44b6ad4e3b9daf8176c42020-11-25T03:41:38ZengElsevierStem Cell Research1873-50612020-08-0147101916Lgr5 maintains stemness and regulates cell property in nasopharyngeal carcinoma through Wnt/β-catenin signaling pathwayFangqi Li0Xiaole Song1Xuewen Li2Xiujuan Zhang3Xiaoyu Feng4Li Wang5Lun Xu6Jiqin Luo7Bijun Zhu8Wenwen Ren9Hongmeng Yu10Yiqun Yu11School of Life Sciences, Shanghai University, Shanghai 200444 ChinaDepartment of Otolaryngology, Eye, Ear, Nose and Throat Hospital, Shanghai Key Clinical, Disciplines of Otorhinolaryngology, Fudan University, Shanghai 200031 ChinaSchool of Life Sciences, Shanghai University, Shanghai 200444 ChinaDepartment of Otolaryngology, Eye, Ear, Nose and Throat Hospital, Shanghai Key Clinical, Disciplines of Otorhinolaryngology, Fudan University, Shanghai 200031 ChinaSchool of Life Sciences, Shanghai University, Shanghai 200444 ChinaDepartment of Otolaryngology, Eye, Ear, Nose and Throat Hospital, Shanghai Key Clinical, Disciplines of Otorhinolaryngology, Fudan University, Shanghai 200031 ChinaDepartment of Otolaryngology, Eye, Ear, Nose and Throat Hospital, Shanghai Key Clinical, Disciplines of Otorhinolaryngology, Fudan University, Shanghai 200031 ChinaDepartment of Otolaryngology, Eye, Ear, Nose and Throat Hospital, Shanghai Key Clinical, Disciplines of Otorhinolaryngology, Fudan University, Shanghai 200031 ChinaDepartment of Otolaryngology, Eye, Ear, Nose and Throat Hospital, Shanghai Key Clinical, Disciplines of Otorhinolaryngology, Fudan University, Shanghai 200031 ChinaDepartment of Otolaryngology, Eye, Ear, Nose and Throat Hospital, Shanghai Key Clinical, Disciplines of Otorhinolaryngology, Fudan University, Shanghai 200031 China; Corresponding authors at: Department of Otolaryngology, Eye, Ear, Nose and Throat Hospital, Fudan University, 83 Fen Yang Road, Shanghai 200031, China.School of Life Sciences, Shanghai University, Shanghai 200444 China; Corresponding authors at: Department of Otolaryngology, Eye, Ear, Nose and Throat Hospital, Fudan University, 83 Fen Yang Road, Shanghai 200031, China.School of Life Sciences, Shanghai University, Shanghai 200444 China; Department of Otolaryngology, Eye, Ear, Nose and Throat Hospital, Shanghai Key Clinical, Disciplines of Otorhinolaryngology, Fudan University, Shanghai 200031 China; Corresponding authors at: Department of Otolaryngology, Eye, Ear, Nose and Throat Hospital, Fudan University, 83 Fen Yang Road, Shanghai 200031, China.Nasopharyngeal carcinoma (NPC) is a common malignant tumor in Southern China and Southeast Asia. In this study, we found that Leucine rich repeat containing G protein-coupled receptor 5 (Lgr5) was highly expressed in NPC tissues and marked NPC stem cells. Lgr5high tumors showed differential transcriptional landscape compared to Lgr5not high tumors. Lgr5 expression was associated with the clinicopathologic features in NPC and was able to regulate the stemness and viability of NPC cell line CNE1 and HNE1. Meanwhile, the migration, invasion and epithelial-mesenchymal transition (EMT) was modulated by Lgr5 via Wnt/β-catenin signaling pathway. Furthermore, Lgr5 could regulate the sensitivity of NPC cells to chemotherapy drugs. Xenografted tumors from Lgr5-overexpressed CNE1 cells showed stronger tumor forming capacity and higher expression level of stem cell markers. Thus, we characterized previously unidentified role of Lgr5 in NPC cells, potential serving as a NPC stem cell biomarker and a therapeutic target against NPC.http://www.sciencedirect.com/science/article/pii/S1873506120302178Lgr5Nasopharyngeal carcinomaStem cellsEpithelial-mesenchymal transitionWnt/β-catenin pathway
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Fangqi Li Xiaole Song Xuewen Li Xiujuan Zhang Xiaoyu Feng Li Wang Lun Xu Jiqin Luo Bijun Zhu Wenwen Ren Hongmeng Yu Yiqun Yu
spellingShingle	Fangqi Li Xiaole Song Xuewen Li Xiujuan Zhang Xiaoyu Feng Li Wang Lun Xu Jiqin Luo Bijun Zhu Wenwen Ren Hongmeng Yu Yiqun Yu Lgr5 maintains stemness and regulates cell property in nasopharyngeal carcinoma through Wnt/β-catenin signaling pathway Stem Cell Research Lgr5 Nasopharyngeal carcinoma Stem cells Epithelial-mesenchymal transition Wnt/β-catenin pathway
author_facet	Fangqi Li Xiaole Song Xuewen Li Xiujuan Zhang Xiaoyu Feng Li Wang Lun Xu Jiqin Luo Bijun Zhu Wenwen Ren Hongmeng Yu Yiqun Yu
author_sort	Fangqi Li
title	Lgr5 maintains stemness and regulates cell property in nasopharyngeal carcinoma through Wnt/β-catenin signaling pathway
title_short	Lgr5 maintains stemness and regulates cell property in nasopharyngeal carcinoma through Wnt/β-catenin signaling pathway
title_full	Lgr5 maintains stemness and regulates cell property in nasopharyngeal carcinoma through Wnt/β-catenin signaling pathway
title_fullStr	Lgr5 maintains stemness and regulates cell property in nasopharyngeal carcinoma through Wnt/β-catenin signaling pathway
title_full_unstemmed	Lgr5 maintains stemness and regulates cell property in nasopharyngeal carcinoma through Wnt/β-catenin signaling pathway
title_sort	lgr5 maintains stemness and regulates cell property in nasopharyngeal carcinoma through wnt/β-catenin signaling pathway
publisher	Elsevier
series	Stem Cell Research
issn	1873-5061
publishDate	2020-08-01
description	Nasopharyngeal carcinoma (NPC) is a common malignant tumor in Southern China and Southeast Asia. In this study, we found that Leucine rich repeat containing G protein-coupled receptor 5 (Lgr5) was highly expressed in NPC tissues and marked NPC stem cells. Lgr5high tumors showed differential transcriptional landscape compared to Lgr5not high tumors. Lgr5 expression was associated with the clinicopathologic features in NPC and was able to regulate the stemness and viability of NPC cell line CNE1 and HNE1. Meanwhile, the migration, invasion and epithelial-mesenchymal transition (EMT) was modulated by Lgr5 via Wnt/β-catenin signaling pathway. Furthermore, Lgr5 could regulate the sensitivity of NPC cells to chemotherapy drugs. Xenografted tumors from Lgr5-overexpressed CNE1 cells showed stronger tumor forming capacity and higher expression level of stem cell markers. Thus, we characterized previously unidentified role of Lgr5 in NPC cells, potential serving as a NPC stem cell biomarker and a therapeutic target against NPC.
topic	Lgr5 Nasopharyngeal carcinoma Stem cells Epithelial-mesenchymal transition Wnt/β-catenin pathway
url	http://www.sciencedirect.com/science/article/pii/S1873506120302178
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Lgr5 maintains stemness and regulates cell property in nasopharyngeal carcinoma through Wnt/β-catenin signaling pathway

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