Isolation of anti-extra-cellular vesicle single-domain antibodies by direct panning on vesicle-enriched fractions
Abstract Background The thorough understanding of the physiological and pathological processes mediated by extracellular vesicles (EVs) is challenged by purification methods which are cumbersome, not reproducible, or insufficient to yield homogeneous material. Chromatography based on both ion-exchan...
Main Authors: | Milica Popovic, Elisa Mazzega, Barbara Toffoletto, Ario de Marco |
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Format: | Article |
Language: | English |
Published: |
BMC
2018-01-01
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Series: | Microbial Cell Factories |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s12934-017-0856-9 |
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