Isolation of anti-extra-cellular vesicle single-domain antibodies by direct panning on vesicle-enriched fractions

Isolation of anti-extra-cellular vesicle single-domain antibodies by direct panning on vesicle-enriched fractions

Abstract Background The thorough understanding of the physiological and pathological processes mediated by extracellular vesicles (EVs) is challenged by purification methods which are cumbersome, not reproducible, or insufficient to yield homogeneous material. Chromatography based on both ion-exchan...

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Bibliographic Details
Main Authors: Milica Popovic, Elisa Mazzega, Barbara Toffoletto, Ario de Marco
Format: Article
Language:English
Published: BMC 2018-01-01
Series:Microbial Cell Factories
Subjects:
Nanobodies
Extracellular vesicles
Panning strategy
Exosomes
Monolith chromatography
Online Access:http://link.springer.com/article/10.1186/s12934-017-0856-9

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http://link.springer.com/article/10.1186/s12934-017-0856-9

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