CABE-RY: A PAM-flexible dual-mutation base editor for reliable modeling of multi-nucleotide variants

CABE-RY: A PAM-flexible dual-mutation base editor for reliable modeling of multi-nucleotide variants

Multi-nucleotide variants (MNVs) represent an important type of genetic variation and have biological and clinical significance. To simulate MNVs, we designed four dual-mutation base editors combining hA3A(Y130F), TadA8e(V106W), and protospacer adjacent motif (PAM)-flexible SpRY and selected cytosin...

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Bibliographic Details
Main Authors: Wanyu Tao, Qing Liu, Shisheng Huang, Xin Wang, Shiyuan Qu, Junfan Guo, Danfeng Ou, Guanglei Li, Yu Zhang, Xiangmin Xu, Xingxu Huang
Format: Article
Language:English
Published: Elsevier 2021-12-01
Series:Molecular Therapy: Nucleic Acids
Subjects:
CRISPR
dual-mutation base editor
MNVs
PAM-flexible
simultaneous A/C conversions
Online Access:http://www.sciencedirect.com/science/article/pii/S2162253121001840

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http://www.sciencedirect.com/science/article/pii/S2162253121001840

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