Isolation and purification of Bacillus thuringiensis var. israelensis IМV В-7465 peptidase with specificity toward elastin and collagen
Peptidase of Bacillus thuringiensis var. israelensis IМV В-7465 was isolated from culture supernatant using consecutive fractionations by an ammonium sulphate (60% saturation), ion-exchange chromatography and gel-filtration on the TSK-gels Toyoperl HW-55 and DEAE 650(M). Specific elastase (442 U∙mg...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
National Academy of Sciences of Ukraine and Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine.
2016-06-01
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Series: | Ukrainian Biochemical Journal |
Online Access: | http://ukrbiochemjournal.org/wp-content/uploads/2016/06/Nidialkova_3_16.pdf |