Isolation and purification of Bacillus thuringiensis var. israelensis IМV В-7465 peptidase with specificity toward elastin and collagen

Peptidase of Bacillus thuringiensis var. israelensis IМV В-7465 was isolated from culture supernatant using consecutive fractionations by an ammonium sulphate (60% saturation), ion-exchange chromatography and gel-filtration on the TSK-gels Toyoperl HW-55 and DEAE 650(M). Specific elastase (442 U∙mg...

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Bibliographic Details
Main Authors: N. А. Nidialkova, L. D. Varbanets, V. O. Chernyshenko
Format: Article
Language:English
Published: National Academy of Sciences of Ukraine and Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine. 2016-06-01
Series:Ukrainian Biochemical Journal
Online Access:http://ukrbiochemjournal.org/wp-content/uploads/2016/06/Nidialkova_3_16.pdf