The presence of residual gold nanoparticles in samples interferes with the RT-qPCR assay used for gene expression profiling

The presence of residual gold nanoparticles in samples interferes with the RT-qPCR assay used for gene expression profiling

Abstract Background RT-qPCR is routinely used in expression profiling of toxicity pathway genes. However, genetic and molecular level studies used to determine, understand and clarify potential risks of engineered nanomaterials (ENMs) are still incomplete. Concerns regarding possible interference ca...

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Bibliographic Details
Main Authors: Natasha M. Sanabria, Mary Gulumian
Format: Article
Language:English
Published: BMC 2017-10-01
Series:Journal of Nanobiotechnology
Subjects:
RT-qPCR
AuNPS
Nanoparticles
Engineered nanomaterials
Toxicology
Risk assessment
Online Access:http://link.springer.com/article/10.1186/s12951-017-0299-9

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http://link.springer.com/article/10.1186/s12951-017-0299-9

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