Optimizing recombinant protein expression via automated induction profiling in microtiter plates at different temperatures

Optimizing recombinant protein expression via automated induction profiling in microtiter plates at different temperatures

Abstract Background Escherichia coli (E. coli) is the most abundant expression host for recombinant proteins. The production efficiency is dependent on a multitude of parameters. Therefore, high-throughput applications have become an increasingly frequent technique to investigate the main factors. W...

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Bibliographic Details
Main Authors: Martina Mühlmann, Eva Forsten, Saskia Noack, Jochen Büchs
Format: Article
Language:English
Published: BMC 2017-11-01
Series:Microbial Cell Factories
Subjects:
Induction profiling
Recombinant protein
Temperature
E. coli
High-throughput
Microtiter plate
Online Access:http://link.springer.com/article/10.1186/s12934-017-0832-4

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http://link.springer.com/article/10.1186/s12934-017-0832-4

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