Expression of Aspergillus nidulans phy Gene in Nicotiana benthamiana Produces Active Phytase with Broad Specificities

Expression of Aspergillus nidulans phy Gene in Nicotiana benthamiana Produces Active Phytase with Broad Specificities

A full-length phytase gene (phy) of Aspergillus nidulans was amplified from the cDNA library by polymerase chain reaction (PCR), and it was introduced into a bacterial expression vector, pET-28a. The recombinant protein (rPhy-E, 56 kDa) was overexpressed in the insoluble fraction of Escherichia coli...

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Bibliographic Details
Main Authors: Tae-Kyun Oh, Sung Oh, Seongdae Kim, Jae Sung Park, Nagarajan Vinod, Kyung Min Jang, Sei Chang Kim, Chang Won Choi, Suk-Min Ko, Dong Kee Jeong, Rajangam Udayakumar
Format: Article
Language:English
Published: MDPI AG 2014-09-01
Series:International Journal of Molecular Sciences
Subjects:
Aspergillus nidulans
phy gene
Escherichia coli
polyclonal antibody
transient expression
Agrobacterium infiltration
oral administration
Online Access:http://www.mdpi.com/1422-0067/15/9/15571

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http://www.mdpi.com/1422-0067/15/9/15571

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