Fishing for newly synthesized proteins with phosphonate-handles

Here the authors describe PhosID, an enrichment strategy using phosphonate-handles, that combines click chemistry and IMAC-based phospho-enrichment for quantitative proteomics analysis of newly synthesized proteins.

Bibliographic Details
Main Authors: Fleur Kleinpenning, Barbara Steigenberger, Wei Wu, Albert J. R. Heck
Format: Article
Language:English
Published: Nature Publishing Group 2020-06-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-020-17010-0