Single-Molecule Tethered Particle Motion: Stepwise Analyses of Site-Specific DNA Recombination

Single-Molecule Tethered Particle Motion: Stepwise Analyses of Site-Specific DNA Recombination

Tethered particle motion/microscopy (TPM) is a biophysical tool used to analyze changes in the effective length of a polymer, tethered at one end, under changing conditions. The tether length is measured indirectly by recording the Brownian motion amplitude of a bead attached to the other end. In th...

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Bibliographic Details
Main Authors: Hsiu-Fang Fan, Chien-Hui Ma, Makkuni Jayaram
Format: Article
Language:English
Published: MDPI AG 2018-05-01
Series:Micromachines
Subjects:
single molecule analysis
tethered particle motion
site-specific recombination
serine recombinases
tyrosine recombinases
Cre
Flp
ϕC31integrase
Online Access:http://www.mdpi.com/2072-666X/9/5/216

Internet

http://www.mdpi.com/2072-666X/9/5/216

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