Bivalent Llama Single-Domain Antibody Fragments against Tumor Necrosis Factor Have Picomolar Potencies due to Intramolecular Interactions

Bivalent Llama Single-Domain Antibody Fragments against Tumor Necrosis Factor Have Picomolar Potencies due to Intramolecular Interactions

The activity of tumor necrosis factor (TNF), a cytokine involved in inflammatory pathologies, can be inhibited by antibodies or trap molecules. Herein, llama-derived variable heavy-chain domains of heavy-chain antibody (VHH, also called Nanobodies™) were generated for the engineering of bivalent con...

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Bibliographic Details
Main Authors: Els Beirnaert, Aline Desmyter, Silvia Spinelli, Marc Lauwereys, Lucien Aarden, Torsten Dreier, Remy Loris, Karen Silence, Caroline Pollet, Christian Cambillau, Hans de Haard
Format: Article
Language:English
Published: Frontiers Media S.A. 2017-07-01
Series:Frontiers in Immunology
Subjects:
tumor necrosis factor
cytokine
inflammation
nanobody
VHH
intramolecular binding
Online Access:http://journal.frontiersin.org/article/10.3389/fimmu.2017.00867/full

Internet

http://journal.frontiersin.org/article/10.3389/fimmu.2017.00867/full

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