CRISPR/Cpf1-mediated DNA-free plant genome editing

Cpf1 is a type V CRISPR effector protein that has different target and guide RNA requirements compared to Cas9, thus offering an addition tool for precision genome editing. Here Kimet al. show that Cpf1 ribonucleoprotein can be introduced into protoplasts and used for transgene-free gene editing in...

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Main Authors:	Hyeran Kim, Sang-Tae Kim, Jahee Ryu, Beum-Chang Kang, Jin-Soo Kim, Sang-Gyu Kim
Format:	Article
Language:	English
Published:	Nature Publishing Group 2017-02-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/ncomms14406

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spelling	doaj-1c3f50a8ce6e434eb8bae032f341edf52021-05-11T07:20:46ZengNature Publishing GroupNature Communications2041-17232017-02-01811710.1038/ncomms14406CRISPR/Cpf1-mediated DNA-free plant genome editingHyeran Kim0Sang-Tae Kim1Jahee Ryu2Beum-Chang Kang3Jin-Soo Kim4Sang-Gyu Kim5Center for Genome Engineering, Institute for Basic ScienceCenter for Genome Engineering, Institute for Basic ScienceCenter for Genome Engineering, Institute for Basic ScienceCenter for Genome Engineering, Institute for Basic ScienceCenter for Genome Engineering, Institute for Basic ScienceCenter for Genome Engineering, Institute for Basic ScienceCpf1 is a type V CRISPR effector protein that has different target and guide RNA requirements compared to Cas9, thus offering an addition tool for precision genome editing. Here Kimet al. show that Cpf1 ribonucleoprotein can be introduced into protoplasts and used for transgene-free gene editing in plants.https://doi.org/10.1038/ncomms14406
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Hyeran Kim Sang-Tae Kim Jahee Ryu Beum-Chang Kang Jin-Soo Kim Sang-Gyu Kim
spellingShingle	Hyeran Kim Sang-Tae Kim Jahee Ryu Beum-Chang Kang Jin-Soo Kim Sang-Gyu Kim CRISPR/Cpf1-mediated DNA-free plant genome editing Nature Communications
author_facet	Hyeran Kim Sang-Tae Kim Jahee Ryu Beum-Chang Kang Jin-Soo Kim Sang-Gyu Kim
author_sort	Hyeran Kim
title	CRISPR/Cpf1-mediated DNA-free plant genome editing
title_short	CRISPR/Cpf1-mediated DNA-free plant genome editing
title_full	CRISPR/Cpf1-mediated DNA-free plant genome editing
title_fullStr	CRISPR/Cpf1-mediated DNA-free plant genome editing
title_full_unstemmed	CRISPR/Cpf1-mediated DNA-free plant genome editing
title_sort	crispr/cpf1-mediated dna-free plant genome editing
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2017-02-01
description	Cpf1 is a type V CRISPR effector protein that has different target and guide RNA requirements compared to Cas9, thus offering an addition tool for precision genome editing. Here Kimet al. show that Cpf1 ribonucleoprotein can be introduced into protoplasts and used for transgene-free gene editing in plants.
url	https://doi.org/10.1038/ncomms14406
work_keys_str_mv	AT hyerankim crisprcpf1mediateddnafreeplantgenomeediting AT sangtaekim crisprcpf1mediateddnafreeplantgenomeediting AT jaheeryu crisprcpf1mediateddnafreeplantgenomeediting AT beumchangkang crisprcpf1mediateddnafreeplantgenomeediting AT jinsookim crisprcpf1mediateddnafreeplantgenomeediting AT sanggyukim crisprcpf1mediateddnafreeplantgenomeediting
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CRISPR/Cpf1-mediated DNA-free plant genome editing

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