CRISPR/Cpf1-mediated DNA-free plant genome editing
Cpf1 is a type V CRISPR effector protein that has different target and guide RNA requirements compared to Cas9, thus offering an addition tool for precision genome editing. Here Kimet al. show that Cpf1 ribonucleoprotein can be introduced into protoplasts and used for transgene-free gene editing in...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2017-02-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/ncomms14406 |