A qPCR Assay to Detect and Quantify Shiga Toxin-Producing E. coli (STEC) in Cattle and on Farms: A Potential Predictive Tool for STEC Culture-Positive Farms

A qPCR Assay to Detect and Quantify Shiga Toxin-Producing E. coli (STEC) in Cattle and on Farms: A Potential Predictive Tool for STEC Culture-Positive Farms

Shiga toxin-producing E. coli (STEC), of various serogroups harboring the intimin gene, form a serious threat to human health. They are asymptomatically carried by cattle. In this study, a quantitative real-time PCR (qPCR) method was developed as a molecular method to detect and quantify Shiga toxin...

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Bibliographic Details
Main Authors: Karen Verstraete, Els Van Coillie, Hadewig Werbrouck, Stephanie Van Weyenberg, Lieve Herman, Jurgen Del-Favero, Peter De Rijk, Lieven De Zutter, Maria-Adelheid Joris, Marc Heyndrickx, Koen DeReu
Format: Article
Language:English
Published: MDPI AG 2014-03-01
Series:Toxins
Subjects:
Shiga toxin
E. coli
real-time PCR
cattle
quantification
intimin
screening
farm
feces
isolation
Online Access:http://www.mdpi.com/2072-6651/6/4/1201

Internet

http://www.mdpi.com/2072-6651/6/4/1201

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