Amplicon-Based, Next-Generation Sequencing Approaches to Characterize Single Nucleotide Polymorphisms of Orthohantavirus Species

Amplicon-Based, Next-Generation Sequencing Approaches to Characterize Single Nucleotide Polymorphisms of Orthohantavirus Species

Whole-genome sequencing (WGS) of viruses from patient or environmental samples can provide tremendous insight into the epidemiology, drug resistance or evolution of a virus. However, we face two common hurdles in obtaining robust sequence information; the low copy number of viral genomes in specimen...

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Bibliographic Details
Main Authors: Mariah K. Taylor, Evan P. Williams, Thidathip Wongsurawat, Piroon Jenjaroenpun, Intawat Nookaew, Colleen B. Jonsson
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-10-01
Series:Frontiers in Cellular and Infection Microbiology
Subjects:
hantavirus
Orthohantavirus
NGS
next generation sequencing
MinION
illumina MiSeq
Online Access:https://www.frontiersin.org/article/10.3389/fcimb.2020.565591/full

Internet

https://www.frontiersin.org/article/10.3389/fcimb.2020.565591/full

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