EVALUATION OF BILE ACIDS-BINDING CAPACITY AND TYROSINASE INHIBITOR POTENCY OF NOVEL PEPTIDES PREPARED FROM AGRICULTURAL WASTES VIA ENZYMATIC HYDROLYSIS

The objective of this study was to prepare bile acidsbinding and tyrosinase inhibitory peptides for industrial potential application. Proteins were extracted from Brassica olercea (cauliflower) midribs, Corchorus olitorius (corchorus) stems and Vicia faba (broad bean) leaves then were subjected t...

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Main Authors:	Sanaa T. El-Sayed, Rehab A. Al- Azzouny, Ola S. Ali
Format:	Article
Language:	English
Published:	Alma Mater Publishing House "Vasile Alecsandri" University of Bacau 2020-06-01
Series:	Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry
Subjects:	bioactive peptides broad bean leaves cauliflower midribs corchorus stems extraction hydrolysate protein digestion ultrafiltration
Online Access:	http://pubs.ub.ro/?pg=revues&rev=cscc6&num=202002&vol=2&aid=5100

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spelling	doaj-1b4385db4f214064bc365576d2a0bbea2020-11-25T02:17:10ZengAlma Mater Publishing House "Vasile Alecsandri" University of BacauScientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry1582-540X1582-540X2020-06-01212141154EVALUATION OF BILE ACIDS-BINDING CAPACITY AND TYROSINASE INHIBITOR POTENCY OF NOVEL PEPTIDES PREPARED FROM AGRICULTURAL WASTES VIA ENZYMATIC HYDROLYSISSanaa T. El-Sayed0 Rehab A. Al- Azzouny1Ola S. Ali2National Research Centre, Biochemistry Department, 60111, Giza, EgyptNational Research Centre, Biochemistry Department, 60111, Giza, EgyptAl-Azhar University, Faculty of Pharmacy, Biochemistry Department, 11751, Cairo, Egypt The objective of this study was to prepare bile acidsbinding and tyrosinase inhibitory peptides for industrial potential application. Proteins were extracted from Brassica olercea (cauliflower) midribs, Corchorus olitorius (corchorus) stems and Vicia faba (broad bean) leaves then were subjected to hydrolysis by immobilized lettuce protease. For maximum peptides production, the influence of incubation temperatures, and substrate concentrations on the hydrolysis processes were investigated. Peptides were fractionated by ultrafiltration into three fractions according to their molecular weight. Corchorus (fractions F2 and F1) and broad bean (fraction F1) peptides showed bile acids-binding higher than that bind by cholestyramine resin. They may have potential application as a cholesterol-reducing agent for hypercholestermic patients. Cauliflower (F2 and F1) and corchorus (fraction F1) peptides showed tyrosinase inhibitor potency higher than that by kojic acid. They may have potential application as a whitening agent. http://pubs.ub.ro/?pg=revues&rev=cscc6&num=202002&vol=2&aid=5100bioactive peptidesbroad bean leavescauliflower midribscorchorus stemsextractionhydrolysateprotein digestionultrafiltration
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Sanaa T. El-Sayed Rehab A. Al- Azzouny Ola S. Ali
spellingShingle	Sanaa T. El-Sayed Rehab A. Al- Azzouny Ola S. Ali EVALUATION OF BILE ACIDS-BINDING CAPACITY AND TYROSINASE INHIBITOR POTENCY OF NOVEL PEPTIDES PREPARED FROM AGRICULTURAL WASTES VIA ENZYMATIC HYDROLYSIS Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry bioactive peptides broad bean leaves cauliflower midribs corchorus stems extraction hydrolysate protein digestion ultrafiltration
author_facet	Sanaa T. El-Sayed Rehab A. Al- Azzouny Ola S. Ali
author_sort	Sanaa T. El-Sayed
title	EVALUATION OF BILE ACIDS-BINDING CAPACITY AND TYROSINASE INHIBITOR POTENCY OF NOVEL PEPTIDES PREPARED FROM AGRICULTURAL WASTES VIA ENZYMATIC HYDROLYSIS
title_short	EVALUATION OF BILE ACIDS-BINDING CAPACITY AND TYROSINASE INHIBITOR POTENCY OF NOVEL PEPTIDES PREPARED FROM AGRICULTURAL WASTES VIA ENZYMATIC HYDROLYSIS
title_full	EVALUATION OF BILE ACIDS-BINDING CAPACITY AND TYROSINASE INHIBITOR POTENCY OF NOVEL PEPTIDES PREPARED FROM AGRICULTURAL WASTES VIA ENZYMATIC HYDROLYSIS
title_fullStr	EVALUATION OF BILE ACIDS-BINDING CAPACITY AND TYROSINASE INHIBITOR POTENCY OF NOVEL PEPTIDES PREPARED FROM AGRICULTURAL WASTES VIA ENZYMATIC HYDROLYSIS
title_full_unstemmed	EVALUATION OF BILE ACIDS-BINDING CAPACITY AND TYROSINASE INHIBITOR POTENCY OF NOVEL PEPTIDES PREPARED FROM AGRICULTURAL WASTES VIA ENZYMATIC HYDROLYSIS
title_sort	evaluation of bile acids-binding capacity and tyrosinase inhibitor potency of novel peptides prepared from agricultural wastes via enzymatic hydrolysis
publisher	Alma Mater Publishing House "Vasile Alecsandri" University of Bacau
series	Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry
issn	1582-540X 1582-540X
publishDate	2020-06-01
description	The objective of this study was to prepare bile acidsbinding and tyrosinase inhibitory peptides for industrial potential application. Proteins were extracted from Brassica olercea (cauliflower) midribs, Corchorus olitorius (corchorus) stems and Vicia faba (broad bean) leaves then were subjected to hydrolysis by immobilized lettuce protease. For maximum peptides production, the influence of incubation temperatures, and substrate concentrations on the hydrolysis processes were investigated. Peptides were fractionated by ultrafiltration into three fractions according to their molecular weight. Corchorus (fractions F2 and F1) and broad bean (fraction F1) peptides showed bile acids-binding higher than that bind by cholestyramine resin. They may have potential application as a cholesterol-reducing agent for hypercholestermic patients. Cauliflower (F2 and F1) and corchorus (fraction F1) peptides showed tyrosinase inhibitor potency higher than that by kojic acid. They may have potential application as a whitening agent.
topic	bioactive peptides broad bean leaves cauliflower midribs corchorus stems extraction hydrolysate protein digestion ultrafiltration
url	http://pubs.ub.ro/?pg=revues&rev=cscc6&num=202002&vol=2&aid=5100
work_keys_str_mv	AT sanaatelsayed evaluationofbileacidsbindingcapacityandtyrosinaseinhibitorpotencyofnovelpeptidespreparedfromagriculturalwastesviaenzymatichydrolysis AT rehabaalazzouny evaluationofbileacidsbindingcapacityandtyrosinaseinhibitorpotencyofnovelpeptidespreparedfromagriculturalwastesviaenzymatichydrolysis AT olasali evaluationofbileacidsbindingcapacityandtyrosinaseinhibitorpotencyofnovelpeptidespreparedfromagriculturalwastesviaenzymatichydrolysis
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EVALUATION OF BILE ACIDS-BINDING CAPACITY AND TYROSINASE INHIBITOR POTENCY OF NOVEL PEPTIDES PREPARED FROM AGRICULTURAL WASTES VIA ENZYMATIC HYDROLYSIS

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